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1741. Patrick 08-07-2022, 02:55
homepage E54. The method of embodiment 52 or 53, wherein the nucleotide analog or analogs are Locked Nucleic Acid (LNA); 2'-O-alkyl-RNA; 2'-amino-DNA; 2'-fluoro-DNA;
arabino nucleic acid (ANA); 2'-fluoro-ANA, hexitol nucleic acid (HNA),
intercalating nucleic acid (INA), constrained ethyl nucleoside (cEt), 2'-O-methyl nucleic acid (2'-OMe), 2'-O-methoxyethyl nucleic acid (2'-MOE), or any combination thereof.
E52. The method of any one of embodiments 16 to 51, wherein the
polynucleotide comprises at least one nucleotide analog.
E51. The method of any one of embodiments 16
to 50, wherein the polynucleotide is an antisense oligonucleotide.
E16. The method of any one of embodiments 1 to 15, wherein the molecule comprises a polynucleotide.
E22. The method of any one of embodiments
16 to 21, wherein the polynucleotide is single stranded.

E37. The method of any one of embodiments 34 to 36, wherein the tolerability category comprises
at least one, at least two, at least three,
at least four, or at least five tolerability categories. E36.
A method of testing or determining in vivo tolerability
of a molecule comprising administering the molecule to a mammal and grading the mammal's
tolerability in a tolerability category. E45.

The method of any one of embodiments 1 to 44,
further comprising measuring tubulin intensity of the molecule in a
culture of neuronal cells.
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36. The antibody or fragment of embodiment 35, wherein the at least a portion of an immunoglobulin constant region comprises an Fc region. Antibody fragments can be made by various techniques, including but not limited to proteolytic digestion of
an intact antibody as well as production by recombinant host
cells. The co-cultured cells were incubated for about 24 hours, and then supernatants were collected for measurement of IFN-.gamma.
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